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Which of the following classes of RNAs is unique to eukaryotes?


A) messenger RNA (mRNA)
B) ribosomal RNA (rRNA)
C) transfer RNA (tRNA)
D) small nuclear RNAs (snRNAs)
E) CRISPR RNAs (crRNAs)

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Below is a list of steps of intron removal and splicing during pre-mRNA processing. Please select the choice that lists the steps in the CORRECT sequential order. 1. Attachment of snRNP U1 to the 5'splice site 2) Transcription of the DNA template into the pre-mRNA molecule 3) Release of lariat structure 4) Splicing together of exons 5) Transesterification reaction at the branch point adenine


A) 1, 2, 3, 4, 5
B) 4, 1, 3, 5, 2
C) 2, 1, 5, 3, 4
D) 3, 5, 1, 2, 4
E) 5, 3, 4, 1, 2

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What do group I and group II introns have in common?


A) Both are found in mitochondrial genes.
B) Both are found in bacteriophages.
C) Both are known to be self-splicing introns.
D) Both are found in protein-coding genes of chloroplasts.
E) Both are frequently found in eukaryotic genes.

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Which class of RNA is MOST abundant in cells?


A) mRNA
B) tRNA
C) rRNA
D) snRNA
E) miRNA

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Which of the following statements CORRECTLY describes the facts about introns and exons?


A) The number of introns is always less than the number of exons in a gene.
B) Introns are degraded in the cytoplasm.
C) All eukaryotic genes contain an intron.
D) Mitochondrial and chloroplast genes do not contain introns.
E) Introns do not contain sequence-specific information.

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What are guide RNAs (gRNAs) and what do they do?

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Guide RNAs (gRNAs) are short synthetic R...

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Which of the following consensus sequences are NOT found in nuclear introns?


A) GU at the 5' splice site at the beginning of the intron
B) AG at the 3' splice site at the end of the intron
C) CCA at the 3' site downstream of the branch point
D) a at the lariat branch point site
E) 3' CAGG consensus sequence at the 3'splice site

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What attributes make miRNA a potent agent for silencing gene expression by allowing it to silence a large number of genes simultaneously?

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MicroRNAs (miRNAs) are small non-coding ...

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Which of the following spliceosomal components specifically recognizes and binds to the branch point of the intron during pre-mRNA splicing?


A) U1
B) U2
C) U5
D) U6
E) spliceosomal proteins

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Compare and contrast the mechanisms of splicing for nuclear pre-mRNA introns, group I introns, group II introns, and tRNA introns.

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Splicing is the process by which non-cod...

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Discuss the features of C. elegans that make it an important model system for studies of animal genetics and development. Include in your answer some unique features of C. elegans compared to other model systems.

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C. elegans, also known as Caenorhabditis...

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What is an snRNP and what role does it play in the cell?

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An snRNP, or small nuclear ribonucleopro...

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Which of the following rRNA components originates from a separate gene transcript rather than as a cleaved product of a long single precursor rRNA transcript?


A) prokaryotic 16S rRNA
B) prokaryotic 23S rRNA
C) eukaryotic 18S rRNA
D) eukaryotic 5.8S rRNA
E) eukaryotic 5S rRNA

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A key modification in the 3' end of eukaryotic mRNA is the addition of 50 to 250 adenine nucleotides, forming a poly(A) tail. Which of the following is NOT a function of the poly(A) tail?


A) The stability of mRNA transcripts in the cytoplasm is affected by the poly(A) tail.
B) The poly(A) tail facilitates the attachment of the ribosome to the mRNA.
C) The poly(A) tail is important for proper nuclear export of the mRNA.
D) The poly(A) tail at the 3' end translates to a long stretch of repeated amino acids.
E) Multiple proteins will recognize and bind to the poly(A) tail in the cytoplasm.

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Suppose a mutation occurred that prevented a eukaryotic pre-mRNA from receiving a 5ʹ cap. What would be an expected result?


A) Transcription would continue past the end of the gene coding sequence resulting in a longer pre-mRNA transcript.
B) Translation would continue past the end of the gene coding sequence resulting in a longer pre-mRNA transcript.
C) Transcription would not occur as the transcription factors would not be able to bind to the promoter.
D) Translation would not occur as the ribosome would not be able to bind to the mRNA.
E) Replication would not occur as DNA polymerase would not be able to bind to the DNA at the origin of replication.

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What would be the MOST likely effect of mutating the consensus sequence found at the 5' splice site of an intron?


A) A shorter than normal protein would be produced.
B) Replication would be inhibited.
C) A longer than normal mRNA would be produced.
D) A longer than normal DNA would be produced.
E) Transcription would terminate prematurely.

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During the posttranscriptional processing of pre-mRNA, a 5' cap is added to an mRNA. Arrange the following steps of the capping process in the CORRECT order. 1. Addition of a guanine nucleotide via a 5'-5' bond 2) Removal of a phosphate from a ribonucleotide triphosphate at the 5' head of the pre-mRNA 3) Methylation at the 2' position of the sugar in the second and the third nucleotides 4) Methylation at position 7 of the terminal guanine base


A) 1, 2, 3, 4
B) 2, 4, 1, 3
C) 4, 1, 3, 2
D) 2, 1, 4, 3
E) 3, 2, 4, 1

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The sequence below represents a pre-mRNA. What would happen if the G in the 5ʹ splice site were mutated to a C? mRNA: 5'ACUGGACAGGUAAGAAUACAACACAGUCGGCACCACG 3'


A) The U2 snRNA would not be able to bind to the branch point because it could not recognize it.
B) The spliceosome complex would be degraded because it could no longer recognize the 5ʹ splice site.
C) The U1 snRNA would not be able to bind complementarily to the 5ʹ splice site.
D) Splicing would still occur appropriately because the G is not essential at the 5ʹ splice site.
E) The 5ʹ cap would not be able to be added because it requires the 5ʹ splice site to be functional.

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Which mechanism allows for the production of polypeptides that are not entirely encoded by DNA?


A) regulated transcription
B) RNA interference
C) alternative RNA processing
D) RNA editing
E) colinearity

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Which mechanism allows for more than one polypeptide to be encoded by a single gene?


A) regulated transcription
B) RNA interference
C) alternative RNA processing
D) self-splicing of introns
E) RNA methylation

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