Question 1

Which is more sensitive, nucleic acid amplification techniques or nucleic acid probe techniques?

Accepted Answer

A)  Nucleic acid probe techniques.
B)  Nucleic acid amplification techniques.
C)  The sensitivities of the two techniques are approximately equal.
D)  It depends on the particular technique as some amplification techniques are more sensitive than others.

Question 2

The three basic steps of polymerase chain reaction (PCR) are denaturation, primer annealing, and primer extension. Which specification listed below is correct?

Accepted Answer

A)  Primer design-oligonucleotide of 15- 30 base pairs
B)  Primer extension-10 minutes during each cycle
C)  Primer annealing-95° C for 1 minute
D)  Denaturation-50° C for 5 minutes
E)  Primer extension-37° C when Taq polymerase is used

Question 3

What is an advantage of nucleic acid sequence based amplification (NASBA) ?

Accepted Answer

A)  It is faster than PCR.
B)  Primers are not necessary.
C)  The reaction is isothermal.
D)  Product can be detected in real time.

Question 4

What technique listed is best to use to detect a mutated tumor gene in a section of tumor tissue?

Accepted Answer

A)  Branched chain PCR
B)  Ligase chain reaction
C)  Nucleic acid sequence based amplification
D)  In situ amplification

Question 5

Most nucleic acid amplification techniques amplify and detect target DNA, but in branched chain detection a labeled probe attaches to the target DNA and the label is amplified for detection.

Accepted Answer

A) True 
 B)False

Exam 13: Amplification Techniques

Most nucleic acid amplification techniques amplify and detect target DNA, but in branched chain detection a labeled probe attaches to the target DNA and the label is amplified for detection.

Correct Answer
verified

What technique listed is best to use to detect a mutated tumor gene in a section of tumor tissue?

Correct Answer
verified

Which is more sensitive, nucleic acid amplification techniques or nucleic acid probe techniques?

Correct Answer
verified

What is an advantage of nucleic acid sequence based amplification (NASBA) ?

Correct Answer
verified

The three basic steps of polymerase chain reaction (PCR) are denaturation, primer annealing, and primer extension. Which specification listed below is correct?

Correct Answer
verified

Exam 13: Amplification Techniques

Most nucleic acid amplification techniques amplify and detect target DNA, but in branched chain detection a labeled probe attaches to the target DNA and the label is amplified for detection.

Correct AnswerverifiedShow Answer

What technique listed is best to use to detect a mutated tumor gene in a section of tumor tissue?

Correct AnswerverifiedShow Answer

Which is more sensitive, nucleic acid amplification techniques or nucleic acid probe techniques?

Correct AnswerverifiedShow Answer

What is an advantage of nucleic acid sequence based amplification (NASBA) ?

Correct AnswerverifiedShow Answer

The three basic steps of polymerase chain reaction (PCR) are denaturation, primer annealing, and primer extension. Which specification listed below is correct?

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
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