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A living,growing culture whose purpose is to maintain a pure culture of an organism indefinitely is a


A) colony
B) stock culture
C) sporulating culture
D) broth culture

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Capnophiles grow best under conditions of


A) high carbon dioxide
B) low carbon dioxide
C) high osmotic pressure
D) low osmotic pressure

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When cells are placed in a hypertonic environment they will undergo ________ .


A) lysis
B) no change in size
C) swelling of the cell which is contained by the cell wall
D) plasmolysis

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Turbidity in a bacterial culture can be measured using a/n


A) incubator
B) colony county
C) spectrophotometer
D) chemostat

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A media on which Gram positive organisms turn green and Gram negative organisms turn blue would be


A) selective
B) differential
C) complex
D) enriched

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Which of the following is not a stage in endospores returning to vegetative state?


A) traumatic agent such as low pH or heat damages the coat
B) outgrowth occurs in a medium with adequate nutrients
C) divisions of the endospores early in their development
D) water and nutrients penetrate damaged coat

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Exoenzymes


A) produced by gram positive rods act in the medium around the organism
B) are released by the golgi apparatus into the cytoplasm where they act
C) produced by gram negative rods act in the periplamic space
D) two of the above

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Imagine NASA sends you a specimen from a planet that they believe contains an extraterrestial bacteria species. You are the microbiologist in charge of determining how to grow the extraterrestial bacteria. Many factors may affect the growth of this extraterrestial bacteria. Choose two and describe how you would test their effects.

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Physical factors that could affect the g...

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Most bacteria reproduce by


A) sexual reproduction
B) binary fission
C) budding
D) homologous recombination

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Serial dilutions are used to


A) slow down the growth of microbes
B) speed up the growth of microbes
C) obtain bacterial cultures at several different concentrations
D) form bacterial colonies

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The streak plate method produces pure cultures by separating individual bacterial cells from one another by


A) dragging them across a solid surface
B) dispersing them in liquid media
C) killing off those cells that are not able to survive in hot agar
D) embedding some cells in the agar so that they are exposed to lower concentrations of oxygen

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A bacterial population grows most rapidly during ________ phase.


A) lag
B) log
C) stationary
D) death

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Which of the following types of organisms would be most likely to have evolved to live in the large intestine,where there is a complete lack of free oxygen?


A) obligate aerobe
B) obligate anaerobe
C) facultative anaerobe
D) aerotolerant anaerobe

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Microbial growth


A) refers to the increase in the size of a microbial cell
B) refers to the increase in the frequency of cell division
C) in a single generation's time leads to double the cell size
D) in a single generation's time leads to double the number of microbes.

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The pour plate method of isolating pure cultures


A) relies on the fact that liquid agar will solidify as it cools
B) is useful for growing microaerophiles
C) results in some organisms growing embedded in the agar
D) all of the above

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In the stationary phase of bacterial growth


A) The number of newly generated cells is greater than the number of dying cells
B) The number of newly generated cells is less than the number of dying cells
C) The number of newly generated cells is the same as the number of dying cells
D) none of the above

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Which of the following statements about bacteria growth is false?


A) agar is used as a solidifying agent in some types of media
B) bacteria growing in a liquid culture will generate colonies
C) a turbid culture is indicative of bacterial growth
D) each bacterium plated will represent a colony-forming unit

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Which of the following statements about media and agar is false?


A) both chocolate agar and blood agar contain blood
B) stock cultures are never directly used for laboratory studies
C) a differential media will allow some bacteria to grow and stop the growth of others
D) a selective media will suppress the growth of some microbes

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Halophiles require an environment with a high concentration of ________ for optimal growth.


A) sugar
B) alcohol
C) salt
D) phosphorous

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Nonculturable organisms


A) are rare
B) can be identified based on their DNA
C) can only be seen on selective agar plates
D) have defined generation times

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