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Once researchers understood that chromosomes are moved by the spindle microtubules,the next question they wanted to answer is how the microtubules function to bring about this process.They used fluorescent labels to make the chromosomes and the microtubular structures fluoresce.When anaphase began (centromeres split) ,they photobleached a section of microtubules.As chromosomes moved toward the poles of the daughter cells,the photobleached sections of the microtubules remained stationary.This result suggests that


A) the microtubules elongate and shorten at the centrosome end.
B) the microtubules overlap and slide with respect to each other,effectively shortening the microtubules without depolymerizing the actual fiber.
C) the microtubules elongate and shorten at their kinetochore end.
D) the microtubules are of constant length;centrosomes move farther apart to separate chromosomes.

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A parent cell divides to form two genetically identical daughter cells in the process of mitosis.For mitosis to take place


A) the parent cell must first be fertilized.
B) the parent cell must replicate its entire genome prior to mitosis.
C) the parent cell must reproduce its DNA during telophase.
D) the parent cell must divide its DNA in half so each daughter cell gets only the genes needed to carry out its functions.In this way,differentiation occurs.

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Cancer-causing mutated tumour suppressor genes result from which of the following?


A) programmed cell death
B) mutations in genes encoding proteins that normally inhibit progression through the cell cycle
C) mutations that cause overexpression of genes encoding proteins that normally stimulate progression through the cell cycle
D) cell-contact inhibition

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Which of the following is NOT a result of mitosis?


A) in fungi,haploid spores are produced from a diploid zygote
B) germination of a new plant from a seed
C) asexual reproduction of haploid yeast cells
D) regeneration of a zebrafish fin
E) all of the above are examples where mitosis occurs

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Regulatory proteins that serve to prevent a cell from entering the S phase under conditions of DNA damage are also known as


A) cyclins.
B) cyclin-dependent kinases.
C) antibodies.
D) tumor suppressors.

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In a culture of cells,it is observed that the cell cycle has arrested (stopped) during the G1 phase.The reason for this could be that


A) not all chromosomes have been replicated.
B) not all components needed for mitosis are present.
C) not all components needed for DNA replication are present.
D) not all chromosomes are attached to mitotic spindles.

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Which cytoskeletal proteins are important constituents of the contractile structures that form the cleavage furrows involved in animal cell cytokinesis?


A) actin
B) dynein
C) tubulin
D) elastin

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Researchers pulsed rapidly dividing cultured cells for 30 minutes with radioactive thymidine.The cells were then exposed to a solution containing non-radiolabeled thymidine.Cells were analyzed at 2-hour intervals.At the 2-hour time point,no cells appeared to be dividing.Only after 4 hours did some labeled cells appear to be in M phase.This result can be explained in the following way:


A) Cultured cells all divide at the same time,and none synthesized DNA during the 30-minute labeling period.
B) The cells were arrested in a nondividing state because of the treatment and could not enter M phase until several hours after the label was removed.
C) Synthesis (S) phase is lengthy-about 12 hours in most cell types-and the radioactive thymidine was not present long enough for most cells to be labeled.
D) There seems to be a gap or a lag in the cell cycle,between the synthesis of DNA and cell division.

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Which of the following is not an effect of mitosis-promoting factor (MPF) involved in moving a cell into M phase?


A) phosphorylation of lamins,initiating breakdown of the nuclear membrane
B) phosphorylation of microtubule associated proteins,triggering the formation of the mitotic spindle
C) phosphorylation of an enzyme that breaks down the cyclin molecule
D) degradation of cyclin-dependent kinase

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DNA is composed of four nucleosides: adenosine,cytidine,thymidine,and guanosine.If scientists introduced radioactive thymidine into the growth medium of the cells,it would be incorporated into the DNA molecule


A) at any point in the cell cycle.
B) when centromeres split so the two chromosomes can be separated.
C) during DNA replication.
D) as the cell enters G₁ of interphase.

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The M-phase checkpoint is designed to make sure all chromosomes are attached to the mitotic spindle.If this fails to happen,in which stage of mitosis would the cells be most likely to arrest?


A) telophase
B) prophase
C) prometaphase
D) metaphase

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Metaphase occurs prior to the splitting of centromeres.It is characterized by


A) aligning of chromosomes on the equator.
B) duplication of centrioles.
C) cytokinesis.
D) disassembly of the nuclear envelope.

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How might spindle microtubules assist in the process of splitting centromeres?


A) the use of motor proteins to split the centromere at specific arginine residues
B) creating tension by pulling toward opposite poles
C) coding for enzymes involved in the process
D) phosphorylating the centromere,which changes its conformation

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What happens when MPF (mitosis-promoting factor) is introduced into immature frog oocytes that are arrested in Gā‚‚?


A) Nothing happens.
B) Fertilization occurs.
C) The cells enter mitosis.
D) Cell differentiation is triggered.

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In the process of chromosome separation,how do microtubules maintain contact with the kinetochores and shorten at the same time?


A) Motor proteins move chromosomes down the microtubular structures of the mitotic spindle.
B) Actin microfilaments cause the microtubular proteins to slide past each other.
C) The centrosomes move apart,so the microtubular proteins do not need to shorten.
D) The centrosomes cause the shortening and depolymerization of the microtubular proteins.

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The term contact inhibition refers to the


A) exchange of signals between cells using cell surface receptions.
B) cessation of cell growth when two cells touch each other.
C) action of non-competitive inhibitors on the catalytic activity of enzymes.

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At which stage does chromosome organization change from diffuse/uncondensed to a compact/condensed state?


A) Prophase
B) Metaphase
C) Interphase
D) Anaphase
E) Telophase

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In human and many other eukaryotic species' cells,the nuclear membrane has to disappear in order for what to take place?


A) cytokinesis
B) attachment of mitotic spindle to kinetochores
C) splitting of the centrosomes
D) disassembly of the nucleolus

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Another term that could be used to describe the process of binary fission is


A) genetic recombination.
B) fusion.
C) recombination.
D) cloning.

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