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Which of the following is true of microbes and their membranes?


A) They are relatively permeable to protons.
B) Protons can leak through directly when the difference in intracellular and extracellular pH is very high.
C) Membrane-permeant organic acids can prevent the leakage of protons.
D) They are able to change the pH optima of their enzymes.
E) Acidophiles use Na+/H+ antiporters to remove internal protons.

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The membrane needs to remain ________ so that it can expand as cells grow larger and so that proteins needed for solute transport can be inserted into the membrane.


A) rigid
B) warm
C) fluid
D) hypertonic
E) polarized

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Which of the following are commonly used to control microbial growth in foods?


A) weak acids
B) strong acids
C) weak bases
D) strong bases
E) enzymes

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A useful biocontrol alternative to antibiotics for treatment of biofilms that can potentially lead to chronic infections is


A) phenolics.
B) high temperatures and pressures.
C) commercial disinfectants.
D) x-rays.
E) bacteriophages.

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Describe the studies with Salmonella that showed that in vitro conditions do not always model what happens in vivo (in this case in the phagocytic vacuole).

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Protein profiles were obtained in vitro ...

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Psychrophiles favor the cold, since their membranes are more fluid at low temperature as a result of the high proportion of ________ present.


A) saturated fatty acids
B) unsaturated fatty acids
C) lipopolysaccharides
D) transport proteins
E) water

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The conditions used in an autoclave may be reproduced at home in


A) a rice cooker.
B) a double-boiler.
C) a microwave.
D) a pressure cooker.
E) boiling water.

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Cyanobacterial blooms such as those shown in the figure below are typically due to human-caused Cyanobacterial blooms such as those shown in the figure below are typically due to human-caused   A)  antibiotic resistance. B)  warming. C)  oxygenation. D)  salinity increase. E)  eutrophication.


A) antibiotic resistance.
B) warming.
C) oxygenation.
D) salinity increase.
E) eutrophication.

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Why is moist heat more efficient at killing microbes than dry heat? How does an autoclave kill microbes so efficiently?

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Moist heat is a more effective killer th...

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Which of the following grows in the indicated pH range shown in the figure below? Which of the following grows in the indicated pH range shown in the figure below?   A)  psychrophiles B)  mesophiles C)  piezophiles D)  alkaliphiles E)  acidophiles


A) psychrophiles
B) mesophiles
C) piezophiles
D) alkaliphiles
E) acidophiles

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How are some organisms able to survive temporary exposures to elevated temperatures?

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Some organisms exhibit heat-sh...

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The use of oxygen as the terminal electron acceptor is called


A) aerobic respiration.
B) anaerobic respiration.
C) fermentation.
D) oxygenic photosynthesis.
E) carbon dioxide fixation.

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Most bacteria require water activity to be greater than


A) 0.10.
B) 0.25.
C) 0.74.
D) 0.91.
E) 1.0.

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Human pathogens are


A) halophilic.
B) psychrophilic.
C) mesophilic.
D) thermophilic.
E) extreme thermophilic.

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What is depicted in the following figure? What is depicted in the following figure?   A)  Antibiotic production has inhibited bacterial growth. B)  A bacteriophage has preyed on the bacterial colonies. C)  Weak acid production has inhibited bacterial growth. D)  A biofilm has become resistant to disinfectants. E)  Starving cells have undergone programmed cell death.


A) Antibiotic production has inhibited bacterial growth.
B) A bacteriophage has preyed on the bacterial colonies.
C) Weak acid production has inhibited bacterial growth.
D) A biofilm has become resistant to disinfectants.
E) Starving cells have undergone programmed cell death.

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What is biocontrol? Describe two examples.

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Answers will vary. Biocontrol is the use...

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During the starvation response, only a limited number of organisms can


A) retool transport systems for the uptake of different nutrients.
B) form metabolically inactive endospores.
C) protect against reactive oxygen species.
D) protect against temperature extremes.
E) protect against pH extremes.

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Why are thermophiles able to survive at temperatures that would quickly kill most mesophiles? Describe at least three adaptations.

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Thermophilic proteins are more stable du...

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Runoff from agricultural fields, lawns, and golf courses can cause an overgrowth of microbes due to


A) eutrophication.
B) starvation response.
C) oligotrophy.
D) antisepsis.
E) pasteurization.

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The fastest growth rate for a species occurs at temperatures where a cell's ________ work most efficiently.


A) lipids
B) membranes
C) nucleic acids
D) proteins
E) polysaccharides

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