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Where would you expect to find a barophile?


A) At the bottom of the ocean
B) In unpasteurized milk
C) In the large intestine
D) In sewage

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In the stationary phase of bacterial growth:


A) the number of newly generated cells is greater than the number of dying cells
B) the number of newly generated cells is less than the number of dying cells
C) the number of newly generated cells is the same as the number of dying cells
D) none of the above

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Most bacteria:


A) exhibit synchronous growth under natural conditions
B) divide when a small new cell develops from the surface of an existing cell
C) do not immediately increase in number when placed in a culture
D) immediately begin to divide when placed in a culture

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Halophiles require an environment with a high concentration of ________ for optimal growth.


A) sugar
B) alcohol
C) salt
D) phosphorous

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Which of the following statements about bacterial growth is false?


A) Agar is used as a solidifying agent in some types of media.
B) Bacteria growing in a liquid culture will generate colonies.
C) A turbid culture is indicative of bacterial growth.
D) Each bacterium plated will represent a colony-forming unit.

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Most bacteria reproduce by:


A) sexual reproduction
B) binary fission
C) budding
D) homologous recombination

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Serial dilutions are used to:


A) slow down the growth of microbes
B) speed up the growth of microbes
C) obtain bacterial cultures at several different concentrations
D) form bacterial colonies

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Sporulation occurs in:


A) Clostridium
B) Staphylocoocus
C) Klebsiella
D) Citrobacter

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This phase decreases at a logarithmic rate. This phase decreases at a logarithmic rate.   A) A B) B C) C D) D


A) A
B) B
C) C
D) D

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Which of the following is most likely to have evolved to live in the deep layers of mud,where there is a complete lack of free oxygen?


A) Obligate aerobe
B) Obligate anaerobe
C) Facultative anaerobe
D) Aerotolerant anaerobe

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A living,growing culture whose purpose is to maintain a pure culture of an organism indefinitely is a _____.


A) colony
B) stock culture
C) sporulating culture
D) broth culture

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A bacterial culture can be kept in the log phase of growth indefinitely with the help of a/an:


A) incubator
B) chemostat
C) spectrophotometer
D) colony counter

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Which of the following counting techniques does not differentiate between live and dead bacterial cells in a culture?


A) Serial dilution
B) Spread plate
C) Pour plate
D) Direct microscopic count

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All of the following are ways microorganisms adapt to limited nutrients except:


A) synthesize increased amount of enzymes for uptake and metabolism of limited nutrients
B) form metabolically active highly resistant endospores
C) synthesize enzymes needed to use a different nutrient source
D) adjust the rate at which they metabolize nutrients

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When a bacterial cell divides into two new cells,the new cells are called:


A) daughter cells
B) sister cells
C) son cells
D) father cells

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Draw and label a standard growth curve. Describe each of the 4 phases. Why do you think bacteria do not continue to double indefinitely? You sample a culture that has reached the fourth phase after 2 days,can you think of one reason why the culture would begin to increase on day 4?

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Growth curve should have a flat lag phas...

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Which of the following bacterial counting techniques relies on a statistical estimate to determine the number of bacteria in a culture?


A) Serial dilution
B) Standard plate count
C) Spread plate
D) Most probable number

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Organisms with special nutritional needs are said to be _____.


A) barophiles
B) fastidious
C) aerobes
D) none of the above

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