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Exam 12: Gene Mutation, DNA Repair, and Homologous Recombination

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Question 31

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If one Holliday junction region is resolved by an NS cut and the other by an EW cut,the resulting chromosomes

A) are nonrecombinant and carry the combinations A₁ and B₂ or A₂ and B₁.
B) are nonrecombinant and carry the combinations A₁ and B₁ or A₂ and B₂.
C) are A₁ and A₂ or B₁ and B₂.
D) are recombinant and carry the combinations A₁ and B₂ or A₂ and B₁.
E) are recombinant and carry the combinations A₁ and B₁ or A₂ and B₂.

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Question 32

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What is the human cancer syndrome associated with inherited p53 mutations?

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Li-Fraumen...

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Question 33

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Given the DNA sequence 5′-TAC AAA ATA CAG CGG-3′,which of these sequences represents a nonsense mutation?

Which type of mutation converts a nucleotide to an alternative structure with the same composition but slightly different placement of hydrogen bonds with a rare,less stable form that causes base-pair mismatch?

If you have screened 100,000 pollen grains and identified 5 mutants,what is the mutation frequency?

Which repair mechanism is an error-prone system that uses bypass polymerases to replicate short segments of daughter strands whose normal replication is blocked by lesions in template strand DNA?

DNA damage signaling processes are essential for regulating the ________ transition within the cell cycle.

You are interested in studying the function of a gene,so you decide to create point mutations to help you identify important residues in your protein. Which of the adenines in the following sequence could you mutate to create a silent transversion mutation? 5′-ATG GGA TGG CAC CTG-3′

What are the two basic types of base-pair substitutions?

Which of these statements best describes gene expression in a damaged cell?

A bacterial culture is exposed to UV radiation and is preparing to repair the DNA damage.Describe two mechanisms of repair used by E.coli to repair UV-induced DNA damage.Would you expect the bacteria to be able to repair more or less of the damage if they were to be placed in a dark environment after UV damage?

A second-site mutation that compensates for the mutation in one gene by mutating a second gene and restoring the wild-type phenotype is also known as a ________ mutation.