Question 1

Most eukaryotic genes are colinear.

Accepted Answer

A)   True
B)   False
C)   It is unknown at this time.

Question 2

Which modifications are typically found on eukaryotic mRNAs

Accepted Answer

A)   Capping only
B)   Addition of poly A only
C)   Capping and poly-A addition
D)   Eukayotic mRNAs are not modified before translation

Question 3

The process of RNA editing can alter the base sequence of the mRNA following transcription.

Accepted Answer

A) True 
 B)False

Question 4

Select the statement that is true regarding tRNA processing in E.coli.

Accepted Answer

A)   RNaseP acts as an endonuclease.
B)   The recognition sequence of RNaseD is 3'CCA5'.
C)   RNaseD acts as an endonuclease.
D)   The action of RNaseP produces the correct 3' end of the mature tRNA.

Question 5

In transcription,the closed complex consists of

Accepted Answer

A)   RNA polymerase.
B)   transcription factors.
C)   double-stranded DNA.
D)   single-stranded DNA.

Question 6

In eukaryotic organisms,the processing of the 45S rRNA into 5.8S,18S,and 28S rRNA occurs where?

Accepted Answer

A)   in the cytoplasm
B)   in the nucleolus
C)   in the endoplasmic reticulum
D)   in the Golgi body
E)   throughout the cell

Question 7

What structural motif is used by sigma factor to bind to DNA?

Accepted Answer

A)   zinc-finger
B)   helix-turn-helix
C)   leucine zipper
D)   homeodomain

Question 8

The unit of transcription at the molecular level is the gene.

Accepted Answer

A) True 
 B)False

Question 9

If an organism is missing the U5 snRNP,it will have trouble performing which step of splicing?

Accepted Answer

A)   Binding of snRNPs to the 5' splice site
B)   Binding of snRNPs to the branch site
C)   Formation of the intron loop
D)   Exon degredation

Question 10

What protein allows RNA polymerase II to interact with transcription factors bound to enhancers or silencers?

Accepted Answer

A)   mediator
B)   TFIIB
C)   TFIID
D)   sigma factors

Question 11

The term ribozyme is given to catalytic RNA molecules.

Accepted Answer

A) True 
 B)False

Question 12

Sigma factor belongs to a general group of proteins called the transcription factors.

Accepted Answer

A) True 
 B)False

Question 13

You are studying transcription in two different unknown systems. Select the experimental questions you could test that would allow you to definitively determine if the system is eukaryotic or prokaryotic.

Accepted Answer

A)   Does transcription in the system require a promoter?
B)   Does RNA capping occur?
C)   Does RNA splicing occur, and if so is it self-splicing?
D)   Does RNA tailing occur?
E)   How many RNA polymerases are present?

Question 14

What process is important for the initiation of translation?

Accepted Answer

A)   alternative splicing
B)   RNA editing
C)   5' capping
D)   base modification

Question 15

What RNA modification is important for mRNA stability?

Accepted Answer

A)   alternative splicing
B)   RNA editing
C)   base modification
D)   3' polyA tailing
E)   processing

Question 16

How many general transcription factors are needed for eukaryotic translation?

Accepted Answer

A)   3
B)   4
C)   5
D)   6

Question 17

What is the name of the transcription start site?

Accepted Answer

A)   promoter
B)   terminator
C)   regulation sequences
D)   transcription factors

Question 18

What do both the rho-dependent and rho-independent mechanisms of termination have in common?

Accepted Answer

A)   terminate transcription immediately after the stop codon
B)   a sequence rich with A-U base pairs
C)   Both require a helicase to separate the DNA-RNA complex.
D)   formation of a stem-loop structure

Question 19

What general transcription factor is a helicase?

Accepted Answer

A)   TFIID
B)   TFIIH
C)   TFIIF
D)   TFIIB

Question 20

Select the eukaryotic regulatory elements.

Accepted Answer

A)   enhancers
B)   silencers
C)   cis-acting elements
D)   Pribnow box

Exam 14: Gene Transcription and RNA Modification

If an organism is missing the U5 snRNP,it will have trouble performing which step of splicing?

Correct AnswerverifiedShow Answer

Select the statement that is true regarding tRNA processing in E.coli.

Correct AnswerverifiedShow Answer

What do both the rho-dependent and rho-independent mechanisms of termination have in common?

Correct AnswerverifiedShow Answer

What structural motif is used by sigma factor to bind to DNA?

Correct AnswerverifiedShow Answer

In transcription,the closed complex consists of

Correct AnswerverifiedShow Answer

What is the name of the transcription start site?

Correct AnswerverifiedShow Answer

Most eukaryotic genes are colinear.

Correct AnswerverifiedShow Answer

Which modifications are typically found on eukaryotic mRNAs

Correct AnswerverifiedShow Answer

What process is important for the initiation of translation?

Correct AnswerverifiedShow Answer

You are studying transcription in two different unknown systems. Select the experimental questions you could test that would allow you to definitively determine if the system is eukaryotic or prokaryotic.

Correct AnswerverifiedShow Answer

What general transcription factor is a helicase?

Correct AnswerverifiedShow Answer

Sigma factor belongs to a general group of proteins called the transcription factors.

Correct AnswerverifiedShow Answer

What RNA modification is important for mRNA stability?

Correct AnswerverifiedShow Answer

The process of RNA editing can alter the base sequence of the mRNA following transcription.

Correct AnswerverifiedShow Answer

The term ribozyme is given to catalytic RNA molecules.

Correct AnswerverifiedShow Answer

How many general transcription factors are needed for eukaryotic translation?

Correct AnswerverifiedShow Answer

What protein allows RNA polymerase II to interact with transcription factors bound to enhancers or silencers?

Correct AnswerverifiedShow Answer

The unit of transcription at the molecular level is the gene.

Correct AnswerverifiedShow Answer

Select the eukaryotic regulatory elements.

Correct AnswerverifiedShow Answer

In eukaryotic organisms,the processing of the 45S rRNA into 5.8S,18S,and 28S rRNA occurs where?

Correct AnswerverifiedShow Answer

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