Question 1

Which of the following might complicate the interpretation of ChIP-Seq data?

Accepted Answer

A)  Because histones can be moved around or ejected from the chromatin, some DNA might not be sequenced if it is not wrapped around a histone
B)  DNA wrapped around nucleosomes can never be sequenced
C)  The regions of DNA wrapped around a histone is too long to be sequenced with current technologies
D)  The DNA between nucleosomes are too compacted to be sequenced

Question 2

When cortisol binds to the glucocorticoid receptor, HSP90 is released.

Accepted Answer

A) True 
 B)False

Question 3

Which of the following recognizes response elements, control elements, and regulatory elements?

Accepted Answer

A)  General transcription factors
B)  Regulatory transcription factors
C)  Mediator
D)  Transactivation domains B

Question 4

What would be the result of a mutation in Hsp90?

Accepted Answer

A)  Glucocorticoid receptor could not form a dimer
B)  The nuclear localization signal would no longer function
C)  Expression of the regulated genes would become constitutive
D)  The hormone would not be able to bind to the glucocorticoid receptor

Question 5

cAMP is known as a second messenger since the pathway is first activated by a extracellular signaling molecule.

Accepted Answer

A) True 
 B)False  True

Question 6

Which one of the following directly interacts with the DNA as a transcriptional regulator?

Accepted Answer

A)  cAMP
B)  G protein
C)  Protein kinase A
D)  CREB protein dimer
E)   Glucocorticoid response element

Question 7

A particular cell contains all of the standard histones but lacks several histone variants. Which of the following MAY be true of this cell?

Accepted Answer

A)  The cell will be completely unable to remodel its chromatin.
B)  The cell will have an enhanced ability to chemically modify its histones.
C)  The cell will be unable to express any of its genes at all.
D)  The cell will express different sets of genes than other cells in the same organism.
E)   The cell will not be able to divide.

Question 8

Which of the following is a region in DNA that binds to a steroid receptor?

Accepted Answer

A)  GRE
B)  IRE
C)  CRE
D)  CREB

Question 9

Genomic imprinting is a result of ___________.

Accepted Answer

A)  Nucleosome location
B)  Histone activation
C)  DNA methylation
D)  Serine to leucine changes in the genetic code C

Question 10

A heterodimer occurs when two identical transcription factors interact on a sequence of DNA.

Accepted Answer

A) True 
 B)False

Question 11

A transactivation domain is an example of a motif found in transcription factors.

Accepted Answer

A) True 
 B)False

Question 12

If a portion of a transcription factor's domain is the same in a variety of organisms, it is called a motif.

Accepted Answer

A) True 
 B)False

Question 13

A mutation in which of the following would result in little or no effect on the expression of a gene regulated by a CRE?

Accepted Answer

A)  G protein
B)  Adenylyl cyclase
C)  Protein kinase A
D)  CREB
E)   Glucocorticoid receptor

Question 14

A particular gene has a mutation in its NFR that causes it to bind abnormally tightly to histones. What effect would you expect this mutation has on the expression of this gene?

Accepted Answer

A)  The expression of the gene would be abnormally high.
B)  The effect on gene expression is impossible to predict.
C)  The gene would encode a different protein instead.
D)  The expression of the gene would be abnormally low or absent.
E)   The promoter of the gene will be constantly available to transcription factors and RNA polymerase.

Question 15

The activity of some transcription factors can be regulated by covalent modifications.

Accepted Answer

A) True 
 B)False

Question 16

DNA that contains actively transcribed genes would most likely contain chromatin in the closed configuration.

Accepted Answer

A) True 
 B)False

Question 17

Which of the following would also work as an enhancer for the following bi-directional enhancer? 5' GTTC 3' 3' CAAG 5'

Accepted Answer

A)  5' GAAC 3' 3' CTTG 5'
B)  5' CTTG 3' 3' GAAC 5'
C)  5' CAAG 3' 3' GTTC 5'
D)  5' CTTC 3' 3' GAAG 5'

Question 18

Which DNA sequence important for gene expression is usually contained within the NFR?

Accepted Answer

A)  Core promoter
B)  CREB binding site
C)  Histone binding site
D)  Transcription termination sequence
E)   Hormone response element

Question 19

Which of the following structural motifs promotes dimerization?

Accepted Answer

A)  Zinc finger
B)  Leucine zipper
C)  Helix-turn-helix
D)  Helix-loop-helix

Question 20

What might happen if the insulator between two genes is missing?

Accepted Answer

A)  Without the insulator, neither gene can be transcribed.
B)  Transcription factors influencing one gene's expression may abnormally influence a neighboring gene's expression.
C)  Transcription factors will not be able to influence the expression of either gene.
D)  Without insulation, both genes will be expressed at maximal levels.
E)   Transcription factors will not be able to bind to the insulator.

Exam 15: Gene Regulation in Eukaryotes I: Transcriptional Regulation

Which of the following might complicate the interpretation of ChIP-Seq data?

Correct AnswerverifiedShow Answer

When cortisol binds to the glucocorticoid receptor, HSP90 is released.

Correct AnswerverifiedShow Answer

Which of the following recognizes response elements, control elements, and regulatory elements?

Correct AnswerverifiedB

What would be the result of a mutation in Hsp90?

Correct AnswerverifiedShow Answer

cAMP is known as a second messenger since the pathway is first activated by a extracellular signaling molecule.

Correct AnswerverifiedTrue

Which one of the following directly interacts with the DNA as a transcriptional regulator?

Correct AnswerverifiedShow Answer

A particular cell contains all of the standard histones but lacks several histone variants. Which of the following MAY be true of this cell?

Correct AnswerverifiedShow Answer

Which of the following is a region in DNA that binds to a steroid receptor?

Correct AnswerverifiedShow Answer

Genomic imprinting is a result of ___________.

Correct AnswerverifiedC

A heterodimer occurs when two identical transcription factors interact on a sequence of DNA.

Correct AnswerverifiedShow Answer

A transactivation domain is an example of a motif found in transcription factors.

Correct AnswerverifiedShow Answer

If a portion of a transcription factor's domain is the same in a variety of organisms, it is called a motif.

Correct AnswerverifiedShow Answer

A mutation in which of the following would result in little or no effect on the expression of a gene regulated by a CRE?

Correct AnswerverifiedShow Answer

A particular gene has a mutation in its NFR that causes it to bind abnormally tightly to histones. What effect would you expect this mutation has on the expression of this gene?

Correct AnswerverifiedShow Answer

The activity of some transcription factors can be regulated by covalent modifications.

Correct AnswerverifiedShow Answer

DNA that contains actively transcribed genes would most likely contain chromatin in the closed configuration.

Correct AnswerverifiedShow Answer

Which of the following would also work as an enhancer for the following bi-directional enhancer? 5' GTTC 3' 3' CAAG 5'

Correct AnswerverifiedShow Answer

Which DNA sequence important for gene expression is usually contained within the NFR?

Correct AnswerverifiedShow Answer

Which of the following structural motifs promotes dimerization?

Correct AnswerverifiedShow Answer

What might happen if the insulator between two genes is missing?

Correct AnswerverifiedShow Answer

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B

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True

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C

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