Question 1

The Prinbow box contains a CACAAC consensus sequence located in the -100 region of the promoter.

Accepted Answer

A) True 
 B)False

Question 2

What kind of promoter is being used in this experiment?

Accepted Answer

A)  Eukaryotic
B)  Prokaryotic
C)  Cannot tell from the information given
D)  Neither eukaryotic nor prokaryotic

Question 3

The consensus sequences are 5'TTGACA3' and 5'TATAAT3' at -35 and -10 respectively. Which of the following would be most easily recognized by  σ\sigmaσ  factor?

Accepted Answer

A)  5'TTGAAA3'
B)  5'TAAATT3'
C)  5'TATATA3'
D)  5'TTCAGA3'

Question 4

What is the purpose of phosphorylating the carboxy terminal domain (CTD) ?

Accepted Answer

A)  Convert from initiation to the elongation stage
B)  To aid in transcriptional initiation
C)  Phosphorylate transcription factors
D)  To aid in promoter recognition

Question 5

What is the correct order of elements that comprise a functional protein encoding gene in a prokaryote?

Accepted Answer

A)   Promoter, regulatory region, transcribed region, terminator
B)   Regulatory region, promoter, transcribed region, terminator
C)   Regulatory region, promoter, terminator, transcribed region
D)   Promoter, transcribed region, regulatory region, terminator

Question 6

Sigma factor belongs to a general group of proteins called the transcription factors.

Accepted Answer

A) True 
 B)False

Question 7

Where does the process of transcription initiate?

Accepted Answer

A)  Promoter
B)  Terminator
C)  Regulation sequences
D)  Transcription factors A

Question 8

What is unusual about the 5' cap found on almost all eukaryotic mRNAs?

Accepted Answer

A)   The nucelotide added is a guanine methylated at N7 and the bond is created between the phosphate group on the guanine and the phosphate on the terminal nucleotide
B)   The nucelotide added is a guanine methylated at N7 and the bond is a typical phosphodiester bond with the terminal nucleotide
C)   The nucelotide added is anadenine methylated at N7 and the bond is created between the phosphate group on the guanine and the phosphate on the terminal nucleotide
D)   The nucelotide added is an adenine methylated at N7 and the bond is typical phosphodiester bond with the terminal nucleotide A

Question 9

The absence of a band when the linker is present at about -25 indicates that

Accepted Answer

A)   This region is critical for the in vitro transcription reaction
B)   this region is not critical for the in vitro transcription reaction
C)   it is not possible to determine if this region is critical for in vitro transcription
D)   the linker interfered with transcription

Question 10

The results obtained when the linker was cloned between -50 and -100 indicate that

Accepted Answer

A)  this region is not critical for transcription but can enhance transcription
B)  the assay failed
C)  when cloned in this region the linkers had a transacting effect
D)  the results of the assay were variable and this is just due to random variation in the assay

Question 11

Basal transcription in eukaryotes involves the action of only the core promoter.

Accepted Answer

A) True 
 B)False

Question 12

The term ribozyme is given to catalytic RNA molecules.

Accepted Answer

A) True 
 B)False

Question 13

The unit of transcription at the molecular level is the gene.

Accepted Answer

A) True 
 B)False

Question 14

The process of RNA editing can alter the base sequence of the mRNA following transcription.

Accepted Answer

A) True 
 B)False

Question 15

What sequences are not considered to be regulatory elements?

Accepted Answer

A)   Enhancers
B)   Silencers
C)   Core promoters
D)   Cis-acting elements
E)   General transcription factors E

Question 16

An R loop experiment was performed on two different mRNA's. The first showed one R loop, while the second showed two R loops separated by a region of double-stranded DNA. What is the composition of these two mRNA's?

Accepted Answer

A)  The first contains no introns and the second two introns
B)  The first contains no introns and the second one intron
C)  The first contains one intron and the second two introns
D)  The first contains two introns and the second one intron

Question 17

The rationale for including the plasmid that was not cloned with the linker was

Accepted Answer

A)   the plasmid without the linker served as a positive control to demonstrate that the assay was performing as expected
B)   that the plasmid was used to make sure there was no RNA degradation
C)   the plasmid was used to make sure there was no DNA degradation
D)   the plasmid was included as a carrier to help separate the RNA that was produced from the unincorporated radioactive nucleotides.

Question 18

What region in eukaryotic genes usually contains the majority of regulatory elements?

Accepted Answer

A)  0 to 50
B)  -50 to 0
C)  -50 to -100
D)  -100 to -150

Question 19

What snRNP holds the exons in place so that they can be connected after the intron is removed?

Accepted Answer

A)  U1
B)  U2
C)  U5
D)  U6

Question 20

In the process of RNA editing adenine or cytosine may be deaminated and the result is

Accepted Answer

A)  Adenine is changed to inosine and cytosine is changed to thymidine
B)  Although both bases are covalenty changed there is no alteration in the tRNAs the modified bases may engage
C)  The new bases effectively result in new codons
D)  Answers adenine is changed to inosine and cytosine is changed to thymidine and the new bases effectively result in new codons are correct (Although both bases are covalenty changed there is no alteration in the tRNAs the modified bases may engage is incorrect) 
E)   Answers adenine is changed to inosine and cytosine is changed to thymidine and although both bases are covalenty changed there is no alteration in the tRNAs the modified bases may engage are correct (The new bases effectively result in new codons is incorrect)

Exam 12: Gene Transcription and RNA Modification

The Prinbow box contains a CACAAC consensus sequence located in the -100 region of the promoter.

Correct AnswerverifiedShow Answer

What kind of promoter is being used in this experiment?

Correct AnswerverifiedShow Answer

The consensus sequences are 5'TTGACA3' and 5'TATAAT3' at -35 and -10 respectively. Which of the following would be most easily recognized by σ\sigmaσ factor?

Correct AnswerverifiedShow Answer

What is the purpose of phosphorylating the carboxy terminal domain (CTD) ?

Correct AnswerverifiedShow Answer

What is the correct order of elements that comprise a functional protein encoding gene in a prokaryote?

Correct AnswerverifiedShow Answer

Sigma factor belongs to a general group of proteins called the transcription factors.

Correct AnswerverifiedShow Answer

Where does the process of transcription initiate?

Correct AnswerverifiedA

What is unusual about the 5' cap found on almost all eukaryotic mRNAs?

Correct AnswerverifiedA

The absence of a band when the linker is present at about -25 indicates that

Correct AnswerverifiedShow Answer

The results obtained when the linker was cloned between -50 and -100 indicate that

Correct AnswerverifiedShow Answer

Basal transcription in eukaryotes involves the action of only the core promoter.

Correct AnswerverifiedShow Answer

The term ribozyme is given to catalytic RNA molecules.

Correct AnswerverifiedShow Answer

The unit of transcription at the molecular level is the gene.

Correct AnswerverifiedShow Answer

The process of RNA editing can alter the base sequence of the mRNA following transcription.

Correct AnswerverifiedShow Answer

What sequences are not considered to be regulatory elements?

Correct AnswerverifiedE

An R loop experiment was performed on two different mRNA's. The first showed one R loop, while the second showed two R loops separated by a region of double-stranded DNA. What is the composition of these two mRNA's?

Correct AnswerverifiedShow Answer

The rationale for including the plasmid that was not cloned with the linker was

Correct AnswerverifiedShow Answer

What region in eukaryotic genes usually contains the majority of regulatory elements?

Correct AnswerverifiedShow Answer

What snRNP holds the exons in place so that they can be connected after the intron is removed?

Correct AnswerverifiedShow Answer

In the process of RNA editing adenine or cytosine may be deaminated and the result is

Correct AnswerverifiedShow Answer

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The consensus sequences are 5'TTGACA3' and 5'TATAAT3' at -35 and -10 respectively. Which of the following would be most easily recognized by $\sigma$ factor?

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A

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A

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E

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