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In order to speed up the sterilization process,which of the following would be useful?


A) Drying the material
B) Washing/mechanical removal of bacteria/organic matter
C) Addition of organics
D) Nothing
E) Washing/mechanical removal of bacteria/organic matter AND addition of organics

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Chlorhexidine


A) is a member of the biguanides.
B) is extensively used in antiseptics.
C) is ineffective against vegetative bacteria.
D) is limited in use due to its high toxicity.
E) is a member of the biguanides AND is extensively used in antiseptics.

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E

Pasteurization


A) is the use of heat to sterilize food products.
B) is the use of heat to reduce numbers of pathogenic/spoilage bacteria in a food item to a safe level.
C) is a process that uses intense cold to kill microorganisms on foods.
D) is a process that uses short bursts of radiation to kill microorganisms on foods.

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To reduce or eliminate disease-/spoilage-causing organisms,food is often subjected to


A) heat.
B) chemical additives.
C) radiation.
D) cold.
E) All of the choices are correct.

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The more bacteria one starts with,the longer it will take to kill them all.

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True

100% ethanol is twice as effective as 60% ethanol at controlling bacteria.

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Which of the following organisms are resistant to destruction by typical disinfection methods?


A) Endospores of Bacillus and Clostridium
B) Pseudomonas
C) Non-enveloped viruses
D) Mycobacterium spp.
E) All of the choices are correct.

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E

Which of the following is not a sterilization method?


A) Hot air oven
B) Autoclave
C) Pasteurization
D) Filtration

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Upon heat treatment,bacteria die at a constant proportion.

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Compounds of tin,mercury,arsenic,and copper are no longer used to prevent microbial growth in cooling water primarily because


A) antibiotics are cheaper.
B) other chemicals were shown to be much more effective.
C) microbes developed resistance to these metals.
D) their use contributes to serious pollution of natural waters.
E) All of the choices are correct.

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Dry heat takes a much shorter time to sterilize material than wet heat.

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A common application of dry heat in the microbiology laboratory is to


A) prepare specimens for study.
B) sterilize media.
C) sterilize plastics.
D) sterilize the inoculating loop.

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One of the first chemicals used by Lister to prevent surgical infections was


A) alcohol.
B) iodine.
C) carbolic acid.
D) mercury.

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Commercial canning processes


A) are geared to destroy Clostridium botulinum spores.
B) kill all endospores.
C) are especially needed on low acid foods.
D) are 12D processes (designed to kill 1012 endospores) .
E) are geared to destroy Clostridium botulinum spores,are especially needed on low acid foods,AND are 12D (designed to kill 1012 endospores) processes.

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Microwaves do not kill organisms directly but kill by


A) the heat they generate in a product.
B) generating free radicals.
C) generating toxins.
D) creating thymine dimers.

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Pasteurization results in the sterilization of food products.

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The autoclave treatment may be monitored by


A) heat-sensitive tape.
B) heat-resistant endospores of Geobacillus stearothermophilus.
C) pressure indicators alone.
D) waiting for contaminants to appear on freshly poured media.
E) heat-sensitive tape AND heat-resistant endospores ofGeobacillus stearothermophilus.

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Generally,membrane filters are not used to remove


A) bacteria from liquids.
B) microorganisms from gases.
C) spoilage agents from alcoholic beverages.
D) enzymes.

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Silver sulfadiazine,a combination of silver and a sulfa drug,is used to


A) disinfect water for drinking.
B) prevent infection of surgical wounds.
C) prevent infection of second- and third-degree burns.
D) counteract lead poisoning.
E) treat bites from werewolves.

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Upon treatment with heat or chemicals,bacteria will


A) all die immediately.
B) die at a constant proportion.
C) die at an exponential rate.
D) die at a geometric rate.

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