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Exam 2: Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis

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Question 31

Multiple Choice

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The purpose of staining cells on a microscope slide is to

A) kill them.
B) secure them to the slide.
C) enlarge the cells.
D) add contrast in order to see them better.
E) see motility.

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Question 32

Multiple Choice

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Which type of microscope achieves the greatest resolution and highest magnification?

A) bright field
B) dark field
C) phase contrast
D) fluorescence
E) electron

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Question 33

Multiple Choice

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Which type of microscope does not use light in forming the specimen image?

Which of the following magnifies the specimen to produce its real image?

A selective medium contains one or more substances that inhibit growth of certain microbes in order to facilitate the growth of other microbes.

Agar is an important component of media because

At the end of the Gram stain,gram-positive bacteria will be seen as red/pink cells.

A common medium used for growing fastidious bacteria is

The bending of light rays as they pass from one medium to another is called refraction.

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Please read the clinical scenario, and then answer the question that follow to become familiar with the traditional NCLEX question format. You begin your shift at the inner city outreach clinic when a young woman enters crying. She is 19 years old and 28 weeks pregnant with her second child. Her complaint is that she woke this morning leaking clear to milky-colored fluid vaginally. Her first child was born 6 weeks early due to premature rupture of membranes and she is worried this is happening again. You reassure the patient, and explain that a vaginal speculum exam will be performed and specimens will be taken for examination. Once obtaining the proper specimens, you label them appropriately, and send the wet mount and culturette to the laboratory for processing. -The patient asks how microbes from her body can be grown in the lab.You explain that specimens are introduced to nutrient medium and that any growth of the microbe that appears after incubating the specimen is called the

The correct microbiological term for the tiny sample of specimen that is put into a nutrient medium in order to produce a culture is the

The procedures for culturing a microorganism require the use of a microscope.

Which type of microscope bombards a whole,metal-coated specimen with electrons moving back and forth over it?

A reducing media is used to culture

Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the specimens.

Which type of media would be the best choice when shipping a sample of bacteria to a laboratory to be tested from a satellite office site?

For which bacterial genus does mannitol salt agar differentiate between species?

All of the following are examples of different types of microbiological media except

An enveloped virus measures 0.02 micrometers ( $\mu$ m) in diameter.What is the diameter of this virus in nanometers (nm) ?