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Which order below reflects the correct procedure for Gram staining?


A) alcohol/acetone,crystal violet,safranin,iodine
B) crystal violet,alcohol/acetone,iodine,safranin
C) crystal violet,iodine,alcohol/acetone,safranin
D) iodine,safranin,crystal violet,alcohol/acetone
E) alcohol/acetone,safranin,crystal violet,iodine

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Alcohol-based compounds can weaken the outer membrane.

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All bacterial cells have


A) one or more chromosomes.
B) one or more fimbriae.
C) the ability to produce endospores.
D) capsules.
E) flagella.

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Which of the following does not pertain to endotoxins?


A) are specific bacterial cell wall lipids
B) can stimulate fever in the human body
C) can cause septic shock in the human body
D) involved in typhoid fever and some meningitis cases
E) found in acid-fast bacterial cell walls

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Which of the following is not a characteristic of a prokaryote?


A) Its DNA is not encased in a membrane.
B) It has a cell wall made of peptidoglycans or other distinct chemicals.
C) It does not have membrane-bound organelles.
D) Its DNA is wrapped around histones.
E) It is unicellular.

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A client has a serious respiratory infection.A random sample of sputum was taken from the patient coughing up blood.The lab technician said they had isolated a bacterium that did not have any peptidoglycan.You hypothesize that the identity of this microbe could possibly be


A) Mycobacterium tuberculosis.
B) Borrelia burgdorferi.
C) Streptococcus pneumoniae.
D) Mycoplasma pneumoniae.
E) Staphylococcus aureus.

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_____ is a macromolecule containing alternating N-acetylglucosamine and N-acetylmuramic acid cross-linked by short peptide fragments.


A) Mycolic acid
B) Outer membrane protein
C) Peptidoglycan
D) Lysozyme

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Endospores of certain bacterial species can enter tissues in the human body,germinate,and cause an infectious disease.

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Which of the following is not true of the outer membrane?


A) The upper most layer is made of lipopolysaccharides.
B) The inner most layer is a phospholipid bilayer.
C) The porin proteins create channels through the outer membrane.
D) Gram-positive bacteria have an outer membrane.
E) The lipid portion of the lipopolysaccharide layer is an endotoxin.

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D

Cells form a _____ arrangement when cells in a chain snap back upon each other,forming a row of cells oriented side by side.


A) tetrad
B) strep
C) staph
D) sarcina
E) palisade

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The prokaryotic cell membrane is made of the same materials as the eukaryotic cell membrane.

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The short,numerous appendages used by some bacterial cells for adhering to surfaces are called


A) flagella.
B) cilia.
C) fimbriae.
D) periplasmic flagella (axial filaments) .
E) sex pili.

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Spirochetes have a twisting and flexing locomotion due to appendages called


A) flagella.
B) cilia.
C) fimbriae.
D) periplasmic flagella (axial filaments) .
E) sex pili.

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D

Peptidoglycan is a unique macromolecule found in bacterial


A) cell walls.
B) cell membranes.
C) capsules.
D) slime layers.
E) inclusions.

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Which of the following is mismatched?


A) methanogens - convert CO2 and H2 gases into methane
B) extreme halophiles - adapted to salty habitats
C) psychrophiles - adapted to very low temperatures
D) hyperthermophiles - adapted to high temperatures
E) thermoplasmas - adapted to frozen environments

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Two major structures that allow bacteria to adhere to surfaces are ___ and ___.


A) pili;ribosomes
B) fimbrae;capsules
C) lipopolysaccharides;techoic acids
D) actin filaments;phospholipid membranes
E) endospores;metachromatic granules

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B

Serological analysis for bacterial identification typically involves using


A) specific antibodies to the bacterial cell antigens.
B) methods to identify cell enzymes.
C) the analysis of the appearance of colonies.
D) a microscope to determine cell morphology.
E) the determination of guanine + cytosine base concentrations.

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You have made a smear of a bacterial culture and have performed the Gram stain on it.Looking at the organism under the microscope,you notice that the cells do not seem to be the dark blue-purple of a gram-positive reaction,but instead are light blue.Your staining procedure was performed correctly.What is your best explanation as to why the bacteria have stained this way?


A) There is something in the wall of the bacteria that has affected the uptake of the crystal violet into the cell wall,thereby staining it improperly.
B) These are mutant bacteria which cannot be stained like other bacteria.
C) The specimen did not undergo heat fixation before staining: therefore,the primary dye does not stick properly to the wall.
D) The bacteria were taken from an inappropriate medium and a chemical is interfering with proper staining of the cells.

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Gram-negative bacteria do not have peptidoglycan in their cell walls.

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Magnetosomes are


A) infoldings of the cell membrane.
B) termed metachromatic granules.
C) responsible for the heat resistance of endospores.
D) composed of magnetic iron oxide particles.
E) found in all bacteria.

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