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Removing genes from molecules of DNA requires the use of special


A) digestive enzymes.
B) restriction enzymes.
C) enzymes from peroxisomes.
D) microscopic scalpels.

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What is the advantage of using restriction enzymes to cleave DNA?

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Restriction enzymes, also known as restr...

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Ian Wilmut successfully cloned Dolly the sheep from a fully differentiated sheep cell.This type of cloning is known as


A) reproductive cloning.
B) therapeutic cloning.
C) stem cell cloning.
D) gene therapy cloning.

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What percent of the human genome is coding DNA?


A) 1 - 1.5%
B) 5 - 7.5%
C) 9.5 - 15%
D) 34 - 55%
E) It changes during a person's life.

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All of these are types of noncoding human DNA except


A) structural DNA.
B) repeated sequences.
C) transposable elements.
D) exons.

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Explain what has to happen to embryonic stem cells from a donor before they are used in a patient as a therapy.

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The idea of cloning animals was presented by _____ in 1938.


A) Thomson
B) Varmus
C) Wilmut
D) Spemann

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In gene transfer procedures,why is it necessary to use processed mRNA molecules to make cDNA to transfer to another organism?

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In gene transfer procedures, it is often...

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Therapeutic cloning successfully addresses the key problem of ________________ that must be solved before embryonic stem cells can be used to repair damaged human organs.


A) immune acceptance
B) shortened life span
C) intra-species transfer of specific genes
D) producing a viable vector

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Explain the techniques used in the cloning of "Dolly."

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One disease in which gene therapy was used,but not successfully yet is


A) the common cold.
B) cystic fibrosis.
C) tuberculosis.
D) anthrax.

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Heat is used in DNA sequencing to _________ the double strands of the molecule.

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Which of the following problems exist when performing human gene therapy?


A) The vector used to carry the gene may elicit a strong immune response,leading to rejection of the added gene.
B) The virus vector may insert its DNA into the middle of a gene which could lead to cancer.
C) The genes carried by the virus vector could be used to produce more viruses.
D) All of the above.
E) The first two are correct.

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Genetic engineering in agriculture has been employed to make crops


A) resistant to insect pests.
B) resistant to certain herbicides.
C) more nutritious.
D) All of these are correct.

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Crop plants are made resistant to insect pests by


A) injecting mRNA for a protein into the roots of the plant.
B) inserting the Bt protein gene into the plant.
C) coating the plant with a gel that acts as a barrier.
D) All of these are correct.

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In therapeutic cloning,why are the embryonic cells used to treat an individual not rejected by that individual?


A) Because the individual's immune system is suppressed with drugs.
B) Because the cells are embryonic.
C) They are rejected,and that is why this has not worked yet.
D) Because the embryonic cells are cloned from the same individual they are injected into.

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A cell is called __________ if it has the ability to form any body tissue or even an adult animal.

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A promising vector is being investigated for use in gene therapy.It is the


A) adenovirus.
B) NIH virus.
C) rhino-associated virus.
D) adeno-associated virus.

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How many genes constitute the human genome?


A) 3 billion
B) 500,000
C) About 25,000
D) 46

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To seal the cut fragments of DNA together,an enzyme called __________ is used.


A) amylase
B) peptidase
C) trypsin
D) ligase

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